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EXPERIMENTAL PLAN |
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Principal Investigator/Program Director Williams, Robert W. |
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Time Line Year 1 Begin processing additional RI strains. Complete set of standard inbred strains. Begin redesign of the database structures using FileMaker Pro. This should be completed in 69 months. After deciding on the structure of several of the databases associated with the MBL, begin to acquire (and reacquire if necessary) images and other data categories (slide coordinates of ROIs in particular). This process will be ongoing for the duration of the project. Explore options for high-level image warehouse systems (e.g., Oracle 8i). Plan suite of image analysis and image enhancement tools. Year 2 Begin processing G10 brains. This should be completed by the end of Year 3. Revamp the interface, mostly to incorporate the data from Projects 2 and 3. Continue development of image database system. Test one or more high-level image warehouse systems. Begin implementing analysis tools. Acquire 1 µm/pixel images of ROIs. Year 3 Finish processing of G10 brains. This should be completed by the end of Year 3. Add through-focus series to the MBL. Continued development of image database system. Implement new high-level image warehouse systems in parallel to existing system (test only). Overlay coordinates of through-focus series on section and ROI images. Add segmentation interface between MBL and NeuroCartographer. Web alpha testing of MBL image analysis Java applets. Acquire 1 µm/pixel images of ROIs. Year 4 Add brains of 100200 aged G10 brains to the MBL. Possible conversion of image database system to robust commercial DB such as Oracle 8i or other image warehouse DB. Web beta testing of MBL image analysis Java applets. Complete all standard and RI strains in the MBL. Acquire 1 µm/pixel images of ROIs. Year 5 Finish development on final MBL image database system. Near final testing of web MBL image analysis Java applets. Consider additional to MBL of a new set of immunohistochemical strains. Finish acquisition of 1 µm/pixel images of ROIs.
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