Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box
























 

 

 

 

 

 

 

 

 

 

 

 

  

 

  
 

RESEARCH PLAN

  
 

Principal Investigator/Program Director Williams,Robert W.

 
  Modern stereology and the iScope project.

We intend to implement an automated process that will generate a large collection of through-focus Z-axis image stacks of each brain in the MBL at very high magnification (<0.8 µm resolution). Each prerecorded stack will require less than 1 MB of storage space. These images will be ideal for unbiased stereological analysis using either direct 3D counting or the optical disector. Two microscopes (one for development, one for production) will be used to generate the stacks. These video clip will be ideal for the high-magnification cell counts that have become a keystone of unbiased stereology (Williams and Rakic, 1988a,b). All high-resolution prerecorded material will also be available on our web and ftp sites and on DV-RAM disks.

Relationship to the Human Brain Project objective.

What about the problems associated with Internet microscopy? One problem at present is that the effectiveness of virtual

Integrative relationship between brain research and neuroinformatics.

What about the problems associated with Internet microscopy? One problem at present is that the effectiveness of virtual

 		 
   
   
   
 

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  Novelty of the Neuroinformatics Component.